Arsenal had to come from a goal down against Leicester before finally making history with a 2-1 victory at Highbury.
The Barclaycard Premiership champions ensured they became the first team this century to go an entire season unbeaten in the top flight after second-half goals from Thierry Henry and Patrick Vieira overturned Leicester's shock lead.
Former Gunner Paul Dickov rose at the far post to head the relegated Foxes in front on 29 minutes but Henry slotted home a penalty two minutes after the break and Vieira (67) rounded goalkeeper Ian Walker before rolling the ball home.
Newcastle beat Aston Villa for fifth place and snatched the coveted UEFA Cup spot thanks to their draw at Liverpool and Manchester United's victory at Villa Park.
Newcastle, who started the day a point behind David O'Leary's side, went ahead against Liverpool through Shola Ameobi's left-foot strike on 25 minutes.
Michael Owen (67) latched onto Steven Gerrard's pass to haul Liverpool level but it was not enough to deny the Magpies a place in Europe.
Aston Villa were beaten 2-0 by visiting Manchester United, who ended the game with nine men.
Cristiano Ronaldo, who was sent off five minutes from the end after picking up his second yellow card, gave the visitors a stunning fourth-minute lead. Ruud van Nistelrooy doubled the advantage six minutes later, slotting home from close range.
Chelsea beat Leeds 1-0 in what could prove to be Claudio Ranieri's last game in charge.
Jesper Gronkjaer, who produced a stunning goal against Manchester United last week, was on target again, scoring in the 20th minute.
Tottenham condemned 10-man Wolves to their seventh home league defeat of the season with a 2-0 victory at Molineux.
Robbie Keane (37) set Spurs on their way and Jermain Defoe capitalised on a woeful back pass from Carl Cort to grab a second on 57 minutes.
Nigerian striker Aiyegbeni Yakubu's four-goal haul against Middlesbrough ensured Portsmouth put a turbulent week behind them as they ran out 5-1 winners at Fratton Park.
Teddy Sheringham, playing his last game for Portsmouth, scored 10 minutes before full-time.
Manchester City ended a disappointing campaign on a high with a thumping 5-1 victory over fellow-strugglers Everton at Eastlands.
City striker Paulo Wanchope (16) scored the opening goal and the Costa Rican pounced again on the half-hour mark.
Nicolas Anelka added third a minute before the break with substitute Kevin Campbell heading Everton's goal on the hour.
Antoine Sibierski and Shaun Wright-Phillips completed the rout with goals in the dying stages.
Andy Cole's 24th-minute goal for Blackburn was equalised by Birmingham's Stern John (83) as the sides drew at Ewood Park.
Charlton beat Southampton 2-1 at The Valley.
Jason Euell netted nine minutes before the interval and Carlton Cole (53) doubled the lead before David Prutton (64) scored what turned out to be a consolation for Saints.
Brian McBride's brace (45, 78) earned Fulham a 2-0 win at Bolton.