The Rev Ian Paisley yesterday said the Ulster Unionist Party displayed "a sorry picture of disharmony and disunity" and accused Mr Trimble of putting his faith in a Prime Minister who had "deliberately deceived the people".
The DUP was a "strong and unified voice" and would use a strategy to "turn the Assembly, planned for our destruction, into an Assembly used to save the Union.
"Unionists must hold their nerve and vote in large numbers for unionist candidates they can trust. This election is a fresh opportunity to elect a strong, decisive, unified unionist voice to advocate the needs of the unionist people and to be an effectual check on republicanism and nationalism," Dr Paisley told a Belfast press conference.
"Mr Trimble has joined the pan-nationalist front by pledging himself with the pan-nationalist front to implement the terms of the agreement. Those who have put their hand to the Belfast Agreement have breached all their previous election promises and they have conceded to the nationalist and republican agenda and must be rejected at the polls."
Dr Paisley said that of the 71 per cent of people who voted for the agreement, 43 per cent were nationalists. The DUP would be "quite happy" to get the vote of every No voter in the Assembly election and he predicted his party would be the majority unionist party in the Assembly.
Such a result would secure for the party and fellow unionists "the power that we need to turn the petard which was manufactured to hang us into a petard to hang those that want to betray the Union."
Asked how he felt about Sinn Fein's Mr Joe Cahill, who is contesting his North Antrim constituency he replied: "He should have been hung up years ago . . ."