Portsmouth players David Nugent and Marc Wilson are understood to have been fined two weeks' wages after a "serious breach of club discipline" on the club's pre-season tour of Portugal.
The pair are reported to have come to blows at the club's hotel in Guimaraes in the aftermath of their 4-0 friendly defeat to Benfica.
A statement from the club said: "David Nugent and Marc Wilson have both been disciplined for a serious breach of club discipline. The matter has been dealt with internally and there will be no further comment from the club."
The pair were immediately sent them home after the incident by Pompey boss Paul Hart.
Nugent, who missed the Benfica friendly with a toe injury, had looked set for a more prominent role at Fratton Park this season following the sale of Peter Crouch to Tottenham.
He has yet to establish himself in the Premier League after a €7million move from Preston in the summer of 2007. He impressed so much in the Championship with the Lancashire club that he earned an England cap in a European Championship qualifier against Andorra in March 2007, scoring a goal in a 3-0 win.
Wilson, 21, made his debut for Pompey in a Carling Cup tie against Chelsea last September. He started two Premier League games for the south coast club and also made one further substitute appearance.
It is another blow for Pompey in what is shaping up to be a difficult campaign for the club.
Chief executive officer Peter Storrie admitted in a private email to a supporter which was leaked to The Sun that the club had “no option but to sell to keep going” should the protracted takeover of Sulaiman Al Fahim be further delayed or fail to materialise at all.
The club have already sold Crouch as well as fellow England international Glen Johnson to Liverpool to help balance the books.
Al Fahim, meanwhile, has reiterated his commitment to buying the club from Alexandre Gaydamak.
A statement on club website www.portsmouthfc.co.uk read: “In response to recent media coverage, the chairman of the board of Portsmouth Football Club, Sulaiman Al Fahim, would like to stress that he remains committed to completing the acquisition of Portsmouth FC as soon as possible, in keeping with the agreement outlined in the Terms Sheet signed in July.”