Tottenham have lodged official complaints over the actions of Manchester United and Liverpool in their pursuits of Dimitar Berbatov and Robbie Keane respectively.
Spurs chairman Daniel Levy has accused United boss Alex Ferguson of "sheer arrogance" and "hypocrisy" over his public pursuit of star striker Berbatov.
And with Liverpool also making clear their interest in Keane, Levy has branded the conduct of both clubs "disgraceful".
The Premier League have acknowledged receipt of the complaints and have written to both accused clubs for their observations before deciding on a course of action.
Ferguson yesterday revealed he had made a bid for Berbatov and said he expected a deal to go through.
Those comments have angered Levy, as have those of Liverpool boss Rafael Benitez, who recently revealed Republic of Ireland record goalscorer Keane was a prime target.
In a statement yesterday on the club's website, www.tottenhamhotspur.com, Levy wrote: "Today's public comments by Manchester United's manager, announcing that he has made an offer for Dimitar and is confident that the deal will go through with time working in their favour, is a blatant example of sheer arrogance and interference with one of our players.
"It is also probably one of the worst offences by any manager in the Premier League to date and is unbelievably hypocritical given his recent comments in respect of Cristiano Ronaldo and Real Madrid.
"This comes after a series of events, dating back to last summer, which have shown Manchester United to be in breach of Premier League rules.
"As a result, we have today made an official complaint to the Premier League about the conduct of Manchester United.
"Benitez made similar comments in respect of Robbie recently and we made an official complaint to the Premier League about the conduct of Liverpool earlier this week.
"The behaviour of both clubs has been disgraceful. We told both clubs very early on that we had no interest in selling Robbie or Dimitar, respectively, and that they should refrain from pursuing the player.
"Both clubs arrogantly chose to ignore this request and we now have evidence that both clubs have systematically been working to prise the players away from us, outside Premier League rules of conduct.
"Our subsequent position has been severely compromised by both clubs making their intentions widely known and indeed making contact with the players and their agents, without the club's permission.
"I have absolutely no wish to sell either player and to date we have not accepted any offer for either."
Benitez and Spurs have clashed before, in March 2006, when the Liverpool boss claimed Spurs were "desperate" to sell Jermain Defoe and take Djibril Cisse.
It led to Benitez's professionalism being questioned by Spurs officials, and the latest fall-out is unlikely to help the relationship between the clubs.
Aston Villa had already been upset this summer by Benitez's comments regarding Gareth Barry's potential move to Anfield.
United have issued a brief response to Tottenham's claims.
A spokesman said: "We are aware of the allegation and will co-operate with the Premier League as necessary."